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Disulfidptosis: a new form of regulated cell death for cancer treatment

Approximately 95% of the oxygen consumed is reduced to water during aerobic metabolism, but the remaining fraction may be converted to reactive oxygen species and other free radicals, inducing oxidative stress

Concentration (start) Volume (start) = Concentration (final) Volume (final) Protocol [1] Monochlorobimane (mCB) is added to the second half liver tissue homogenate to a final concentration of 100 mM along with glutathione S-transferase (1 U/mL) obtained from equine liver

This results in endoplasmic reticulum stress, lipid peroxidation, and ferroptosis, subsequently reducing TNF- and IFN- production and increasing PD-1 expression [139]