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In general, here are a few tips for bulking up your recipes without relying heavily on carbs: Add inexpensive greens like cabbage, frozen spinach, or chopped kale

When cultured in YP20D medium supplemented with 14 mM acrolein, the parental KACC 48331 strain failed to grow, whereas 21 out of 376 UV-induced mutants exhibited detectable growth

Signal Transduct Target Ther 8:267 Wu D, Re D, Nagai M et al (2006) The inflammatory NADPH oxidase enzyme modulates motor neuron degeneration in amyotrophic lateral sclerosis mice

Following electroporation, cells were incubated for 48 h in recovery medium (RPMI-1640 supplemented with 100 non-essential amino acids (Gibco) and 1 mM sodium pyruvate (Gibco)), followed by sorting for GFP (BD FACS Aria III)