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doi: 10.1371/journal.pone.0147568 145 TiwariB.KalimS.BangarP.KumariR.KumarS.GaikwadA.et al

After another centrifugation step for 15 min at 20,000 g and 4 C, 5x Laemmli buffer containing 10% -mercaptoethanol (-Me) was added to the samples

Integrated metabolomic and transcriptomic analyses reveal the roles of Alanine, aspartate and glutamate metabolism and glutathione metabolism in response to salt stress in tomato

Upon staining, the fluorescent product generated can be visualized using a wide-field fluorescence microscope equipped with a standard orange (e.g., 550/620 nm) fluorescent cube, or cytometrically using any flow cytometer equipped with a blue (488 nm) laser