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Given that both IGF-I and IGFBP-3 are rapidly cleared from the circulation if unbound, using the IGF-I/IGFBP-3 ratio and disregarding IGF-II concentrations (that are 2-3-fold those of IGF-I on a molar basis) does not make any sense

Another subtle mistake is relaxing the muscle incorrectly: for an intramuscular injection, you want the target muscle loose, not flexed, because a tense muscle resists the needle and amplifies pain

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