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Primer sequences and annealing temperatures, for Claudin-2 (CLDN-2), Junctional Adhesion Molecule 1 (JAM-1), Occludin (OCLN), Zonula Occludens-1 (ZO-1), Claudin-4 (CLDN-4), Ribosomal Protein Lateral Stalk Subunit (P0-RPLP0), Mucin-1 (MUC-1), Mucin-2 (MUC-2), Mucin-5AC (MUC-5A) cyclin-dependent kinase inhibitor 1 (p21) and tumor protein p53 (p53), are listed in Supplementary Table 1

However, it may cause side effects such as fatigue, loss of appetite and nausea

Typically anywhere between 30-45 minutes

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