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The glutaminase activity in L-asparaginase is believed to exert anti-cancer effect on ASNS-expressing cells [47]

However, the transition of these technologies from basic research to clinical application still faces substantial obstacles, the most prominent of which is the unresolved issue of off-target effects

For total DNA staining, membranes were incubated in 0.1% (wt/vol) methylene blue solution containing 0.5 M sodium acetate (pH 5.2) overnight at room temperature