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doi: 10.1152/ajpregu.1999.276.4.R929

Incorporation of D-Ala2 in growth hormone-releasing hormone-(1-29)-NH2 increases the half-life and decreases metabolic clearance in normal men

Then, collected the supernatant which containing cardiomyocytes and transferred into another culture dish with Dulbeccos modified Eagles medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Gibco) for 24 h