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2011, Heidelberg: Springer Science+Business Media, LLC, 205-230.Hsp70: Anti-apoptotic and tumorigenic protein, Methods in Molecular Biology Proctor CJ, Lorimer IA: Modelling the role of the Hsp70/Hsp90 system in the maintenance of protein homeostasis

The samples were lysed by freezing and thawing (three times), followed by centrifugation at 13,000 g for 10 min, and the supernatant was collected for subsequent assays