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10.1016/j.bbi.2018.12.013 136 MechaM.Yanguas-CassN.FeliA.MestreL.Carrillo-SalinasF

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The inoculated assay plates were incubated anaerobically at 37 C for 2024 h, then processed as described above under Crystal violet biofilm assay, except that the wells were not imaged prior to fixation and OD 600 readings were also not taken, as for the purposes of the high-throughput primary screen, we did not include replicate wells that could be resuspended, although we did note occasional wells with a complete failure to grow and omitted those from further analysis

The team had been working on it quietly for some time and began shipping it in the summer of 2021