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49,119 Over the past 30 years, the widespread use of X-ray and Cryo-EM has facilitated the characterization of GPCR-orthosteric ligand complexes, with 657 class A, 16 class B1, 6 class B2, 19 class C, 18 class F, and 1 class T structures solved (supplementary Table 15)

For ribosome fractions, target bands were normalized to the intensity of the corresponding RPS6 (eS6) band in the same lane, whereas for whole-cell extracts, normalization was performed against the GAPDH band