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Each strain was grown overnight at 37 C in 2 mL of LB supplemented with 0.5% glucose and 10 g/mL gentamicin, then diluted 1:100 into 100 mL LB supplemented with 10 g/mL gentamicin and grown at 37 C for 30 min

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These potential therapeutic strategies may include the development of new drugs to regulate iron homeostasis, intervention in specific pathways of ferroptosis, and research on how to improve the treatment outcomes of joint diseases by regulating the expression of proteins related to iron metabolism