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Plasmids originated from the pFL44L-based library were extracted and purified with the Zymoprep kit (Zymo Research), amplified in Escherichia coli and then retransformed into the yeast strain overexpressing CYS4 to confirm their ability to reverse methionine auxotrophy

1 Introduction The human microbiome encompasses the collective genetic material of microorganisms residing within us, including protozoa, archaea, eukaryotes, viruses, and primarily bacteria, which coexist symbiotically on and inside various regions of the human body

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