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Cladribine, gemcitabine, lovastatin and tamoxifen have an inhibitory effect on melanoma cell lines in vitro, also in combination with conventional targeted kinase inhibitors To identify the most promising drug candidates for in vitro testing, we filtered the predicted drugs based on their predicted efficacy, prior evidence from high-throughput CRISPR [34] and drug screens [35], IC 50 values in melanoma and other cancer types, known metabolic targets, mechanisms and availability of clinical trial data in phase II or higher (Table 2 and Supplementary File 2)

We used AUROC tests of equality to identify statistical differences between case definitions

In the present study, multiple-alignment of APX sequences revealed that chloroplastic/mitochondrial-related APXs contained the transit peptide residues in approximately 190 amino acids from the N-terminal site while cytosolic APXs did not have any putative transit peptide (Supplementary Figure S4)