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Measurement of SIRT1 and SIRT3 activities in cultured cells and in mouse tissues Cultured CHO-mCD38 cells, and liver and spleen from treated mice were harvested and homogenized in M-PER Mammalian Protein extraction buffer on ice as described previously 25

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Thus, the number of participants are lower than the number who completed the clinical trials in most cases since blood was not always obtained from every participant at the end of the trial for various reasons, including the participant refusal