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Role of divalent metal cations in ATP hydrolysis catalyzed by the hepatitis C virus NS3 helicase: magnesium provides a bridge for ATP to fuel unwinding

Ultra-thin sections (70 nm thick) were obtained using an RMC Ultramicrotome equipped with Diatome diamond knives (PowerTome, USA), mounted on 200 mesh copper grids (FCF200-NI-50, Electron Microscopy Sciences), and contrasted with uranyl acetate and 3% lead citrate for 5 min each