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They were then co-transfected in the presence of polyethylenimine (Sigma-Aldrich, 408727) with 6 g of the plasmid of interest and 6 g of the packaging plasmids pCMV-VSV-G (Addgene, 8454) and pCMVdR8.2 dvpr (Addgene, 8455) in a 1:8 ratio