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Treatment of cells Hepatoma HepG2 cells were maintained in DMEM (GE Healthcare, Buckinghamshire, UK) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 g/ml streptomycin at 37 C in 5% CO 2

Our strains are sourced and cultivated from a specialist probiotic manufacture

In this context, lifestyle, gender, drugs, age, and microbiota have a synergistic impact on the levels of TMAO ( Figure 2 The mechanism of TMAO leading to CVDs The mechanism of TMAO resulting in atherosclerosis, HF, and hypertension has gradually been clarified ( Supplementary Table 3 )

However, there are currently no suitable medicines or agonists that can directly induce GPX3 expression, and many researchers have constructed overexpression plasmids to increase GPX3 expression in cells [50]