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Even if youre purifying the same protein over and over, it is probably still a good idea to clean and regenerate your column about every five purifications, or earlier if you notice that the binding capacity of your beads is getting worse

However, related research is lacking

Complementarily, the use of preclinical assays that are not based on specific mutations models should be considered, as for example the use of iPSCs from sALS and fALS patients (with or without specific mutations) that can be differentiated in MNs, astrocytes, and microglial cells [278] and represent a valuable tool for screening different compounds [279]

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