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Heat inactivated serum was included as negative control

There are also supportive conceptual points providing a larger therapeutical background [24,25]

2 Mapping cerebellar circuits: anatomical insights into pain processing 2.1 Cerebrocortical and cerebellar contributions to pain modulation Pain is a multidimensional experience arising from the interplay of sensory, affective, cognitive, and social factors (20), processed through distributed brain networks that include the thalamus, brainstem nuclei, somatosensory cortices, and prefrontal regions (21)

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