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In recent years, LPs have been used as carriers of therapeutic or imaging agents for the treatment or diagnosis of disorders in the central nervous system (CNS) [16,17,18]

This damage eventually leads to apoptosis and necrosis of the neurons (Nicklas et al., 1985

They were further incubated with secondary antibodies and streptavidinhorseradish peroxidase with diaminobenzidine.The resulting images were captured using an Axio Observer 7 microscope (Zeiss)