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These suggested that the regulation of ferroptosis by hypoxia may depend on the expression of other genes involved in ferroptosis, which will be differently expressed in tumor and normal cells

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To validate this hypothesis, SIRT2 activity was suppressed by shRNA or inhibitor SirReal2 in U2OS cells stably overexpressing Flag-GCLC, followed by the treatment with 700 M TBH for 12 h