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1 Introduction Melanoma is the most aggressive type of skin cancer, with a highly metastatic potential in 3.5% of cases ( The composition of the BCG cell wall is highly immunogenic ( 2 ) and hexamannoside (PIM 6 ) phosphatidylinositol mannosides ( Mycobacterium tuberculosis which elicit a TLR4-mediated response ( BCG also produces moderate amounts of cyclic di-AMP, capable to activate Stimulator of interferon genes (STING) ( A previous study from our group showed that BCG acts modulating the TME in subcutaneous MB49 bladder tumor model through the recruitment of antitumor cells, such as inflammatory macrophages, neutrophils, CD8 + T cells and NKT cells