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Precipitation of protein from THP-1 cell conditioned media, followed by Western blotting analysis showed similar amounts of IL-1sRII in the supernatants of unstimulated and LPS-treated cells (Fig

Disease-driving CD4+ T cell clonotypes persist for decades in celiac disease

( b ) Proliferation of cells grown in the presence of serum and growth factors was determined using the WST-1 assay (data are from one experiment that was independently repeated two times with similar results (Supplementary Table 1))