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These hypoxic and acidic regions function as spatial refuges in which genetically and phenotypically diverse tumor cells persist, reinforcing both pre-existing and adaptive resistance mechanisms and setting the stage for relapse once selective pressure is relieved

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the negative control should appear as a healthy growing culture by microscopic inspection

Guo Q, Zheng Y, Shi J, Wang J, Li G, Li C, Fromson JA, Xu Y, Liu X, Xu H, Zhang T, Lu Y, Chen X, Hu H, Tang Y, Yang S, Zhou H, Wang X, Chen H, Wang Z, Yang Z (2020) Immediate psychological distress in quarantined patients with COVID-19 and its association with peripheral inflammation: a mixed-method study