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[DOI] [PMC free article] [PubMed] [Google Scholar] 2.Andrade Silva M., da Silva A., do Amaral M.A., Fragas M.G., Cmara N

Mutations were inserted through site-directed mutagenesis using the Quick Change II Site Directed Mutagenesis Kit (Stratagene)

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Selection based on preliminary experiments For example, when the researchers selected the dose to be administered for SRS16-86, five groups were modeled as sham group, SCI group, SCI + 5 mg/kg SRS16-86 group, SCI + 10 mg/kg SRS16-86 group and SCI + 15 mg/kg SRS16-86 group, and then tested motor recovery 2 weeks after SCI to select a dose of 15 mg/kg SRS16-86 (Zhang Y