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Before commencing any new supplement regimen, always consult a healthcare professional

To date, vitamin B12 is officially included as supplement to pemetrexed treatment (a chemotherapeutic used in pleural mesothelioma and non-small cell lung cancer because of its folate similarity and inhibition of purine and pyrimidine synthesis)

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To this end, we used a high-copy plasmid genomic DNA library to perform an unbiased genome-wide suppressor screen 44 : We transformed the VMA2-AID* cells with 1588 overexpression plasmids from the library and examined the induction of V-ATPase-dependent autophagy in each transformant by using the western blot-based GFP-Atg8 processing as the readout assay