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doi: 10.3389/fphar.2025.1490480 Received 03 September 2024 Accepted 01 April 2025 Published 16 April 2025 Volume 16 - 2025 Edited by Jae-hyeon Park, National Institute on Aging (NIH), United States Reviewed by Rajbir Singh, Patanjali Research Institute, India Naveen Sharma, Chung-Ang University, Republic of Korea Updates Copyright 2025 Wang, Wei, Chen and Chen

The 1-cm-long root segments were incubated in 10% KOH at 90C for 50 min, bleached by 10% H 2 O 2 for 15 min, acidified by 0.2 mol/L HCl for 10 min, and stained by 0.05% trypan blue in lactophenol for 30 s

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