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To prevent graft rejection, all animals received immunosuppression with cyclosporine A injections (30 mg/kg) starting one day before surgery, followed by injections every other day at 10 mg/kg

Higher risk skin types People with Fitzpatrick skin types IVVI (medium to deep brown skin tones) are more likely to develop PIH

After fixation, conjugates were stained with mouse anti-Xpress plus a secondary Alexa fluor 488-coupled Ab (AF488) and counterstained with DAPI