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There is nothing wrong with this approach, as it follows the regulatory framework

The reaction was then quenched by adding 50 L of 10% (w/v) sodium dodecyl sulfate (SDS)

Located in the plasma membrane, scramblases are involved in the Ca 2+ -dependent distribution of phospholipids (scrambling) ( Knock-down of PLSCR1 in RBL-2H3 cells significantly impaired FcRI-mediated degranulation and release of vascular endothelial growth factor ( In vivo studies using Plscr1 / mice showed reduced FcRI-dependent passive systemic anaphylaxis and serum histamine levels compared to wild-type mice ( Plscr1 / animals, indicating that PLSCR1 also counter-regulates IgE-dependent anaphylaxis at later stages

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