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Aruoma OI, Whiteman M, England TG, Halliwell B (1997) Antioxidant action of ergothioneine: assessment of its ability to scavenge peroxynitrite

All compounds were dissolved in dimethyl sulfoxide (DMSO) and diluted with sterilized distilled water until the concentration of DMSO was 1% for 30 min in a 50 l reaction mixture containing 50 mM Tris-Cl (pH 8.0) and 5 mM DTT

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Hyperglycemia itself fuels ROS production, hexosamine-pathway flux, and NF-B activation in macrophages, sustaining chronic inflammation 368,369