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In addition, infected cells were selected by using 2.5 g/ml puromycin (MCE, USA) for 14 days, and the transfection efficiency was verified by RT-qPCR and Western blot analysis

For each sample, 25 l of liposomes were mixed with 25 l of the 2 x reaction buffer consisting of 10LM [ 3 H]GSSG (Figure 3a and Extended Data Figure 4l) or 40 M [ 3 H]GSSG (Extended Data Figure 7F), 150 mM KCl, and 50 mM HEPES (pH 7.4)

Oxidized bases are usually recognized and repaired by the base excision pathway (BER)

Taken together, present study represents a detailed identification of DJ-1/PfpI domain containing proteins in complete plant kingdom