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Recombinant fusion proteins comprising a polypeptide of the invention fused to a polypeptide sequence that dimerizes or trimerizes in solution are expressed in suitable host cells, and the resulting soluble multimeric fusion protein is recovered from the culture supernatant using techniques known in the art

For the phi subclass, we found that two transcripts ( Pav_sc0001124.1_g450.1.mk (PavGST25), Pav_sc0000848.1_g720.1.mk (PavGST60) were more highly expressed in the 80H and 100 H while Pav_sc0001215.1_g320.1.mk (PavGST26) , and Pav_sc0007796.1_g150.1.mk (PavGST16) expressed slightly positive expression pattern as compared to control (Figure 12)

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There was no difference in IL-2 among the three groups