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5 l of metabolites suspension was loaded and resolved on an Acquity UPLC BEH C18 column (1.7 m, 2.1 100 mm, Waters) using mobile phases of water with 0.1% formic acid (buffer A) and acetonitrile with 0.1% formic acid (buffer B) with a flow rate of 0.3 ml/min and 8 minutes long gradient

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Testing is recommended when other vitamin deficiencies are ruled out, as biotin works synergistically with other b vitamins to promote strong hair and healthy follicle function