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After confirming a surgical level of anesthesia, the thoracic cavity was opened, and blood was collected from the heart

Additionally, 1 H- 15 N heteronuclear single quantum coherence (HSQC) NMR experiments were conducted to verify whether p53 TAD PEP effectively inhibited the interaction between FOXO4 FHD and p53 TAD

The cells were spun down and washed once with 50 mM Tris-HCl (pH 7.5), then treated with 400 g/mL RNase A for 34 h, at 37 C

however, it is important to carefully investigate aggravating and triggering factors by obtaining a detailed history of the patient (Table 1) and to rule out other pigmentary disorders, which can mimic melasma