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R., Palmer, R

Besides, a 200-times permutation was conducted to validate the OPLS-DA model against over-fitting, and variable influence on projection (VIP) values of each metabolite in the OPLS-DA model was calculated

Inhibition of 5-LOX in a Cell-Free Assay The enzyme activity of human recombinant 5-LOX was determined spectrophotometrically by monitoring the oxidation of H 2 DCFDA to the highly fluorescent 2,7-dichlorofluorescein during the catalytic reaction mediated by 5-LOX as reported (Pufahl et al., 2007)

The concentration of Cys in the human body is determined by the process called transsulfuration, in which homocysteine (Hcy) (Fig