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Identification of targets of sulforaphane acting on the ASD and PPI network analysis A total of 275 targets associated with sulforaphane and 7,986 targets associated with ASD were identified following the exclusion of duplicates (Table S2)

doi:10.1016/j.ymgme.2010.11.160 [CrossRef] 2

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Injected oocytes were stored at 18 C in ND96 storage buffer (96 mM NaCl, 2 mM KCl, 1 mM MgCl 2 , 1.8 mM CaCl 2 , 5 mM hemisodium-HEPES, pH 7.4 supplemented with 50 g/ml gentamycin, 2.5 mM sodium pyruvate, 50 g/mL tetracycline and 0.5 mM theophylline) for three days to allow the expression of hOCTN2 on the oocyte plasma membrane