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For live imaging of oocysts and visualisation of acidic compartments in the oocyst, the midgut was dissected into PBS containing Lysotracker Deep Red (1 M, Invitrogen) or Lysotracker Green DND-26 (1 M, Invitrogen) along with Hoechst and incubated for 10 min at RT before imaging with a Zeiss Axio Imager 2 fluorescent microscope

CrossRef Metabolic Risk/Epidemiology Gestational Diabetes Mellitus and Its Implications across the Life Span Brandy Wicklow, Ravi Retnakaran Diabetes Metab J

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3164-A SPLEEN STIFFNESS AS A NON-INVASIVE TEST TO EVALUATE ESOPHAGEAL VARICES NEEDING TREATMENT AND PORTAL PRESSURE GRADIENT IN LIVER CIRRHOSIS Jeong-Ju Yoo, Soonchunhyang University Bucheon Hospital, Sang Gyune Kim, Soonchunhyang University Bucheon Hospital, Soonchunhyang University College of Medicine, Bucheon, Republic of Korea and Young Seok Kim, Soon Chun Hyang University Background: One of the complications of liver cirrhosis is an increase in the stiffness of the spleen, which is thought to be related to changes in blood flow within the liver and the spleen