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Autoimmune-associated PTPN22 R620W variation reduces phosphorylation of lymphoid phosphatase on an inhibitory tyrosine residue

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OCR levels were normalized on total protein content (g), and the activities of the complexes were calculated using the following OCR values: Complex I: OCR basal (Measurement 2) - OCR after rotenone (Measurement 4) Complex II: OCR after succinate (Measurement 6) Complex IV: OCR after ascorbate/TMPD (Measurement 10) Uncoupling assay Cells were plated 2 days before the assay, following the protocol of complex activities described above