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These effects are not well-established in controlled human trials and should be considered speculative at this stage

When autophagy is initiated, an isolated membrane structure called a phagophore is formed at a special region associated with the endoplasmic reticulum (ER), Golgi apparatus, and ERGolgi intermediate compartment 20,21,22

doi: 10.1016/j.cell.2022.06.003 162 BeharierOTyurinVAGoffJPGuerrero-SantoroJKajiwaraKChuTet al

As for the best treatment duration, in an open-label trial GlyNAC supplementation for 16-weeks or 24-weeks resulted in similar outcomes, while withdrawing GlyNAC for 12-weeks led to loss/decrease of benefits