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Advanced concept box The porphyrias Defects in the heme synthetic pathway lead to rare disorders known as porphyrias

HRMS [M + H] (C 14 H 14 N 3 O 2 ): Calculated: 256.1086

In this study, all of the solvents attained their maximal extraction after 60 min, and when the extraction time was extended, the recovery decreased, most likely due to heat degradation (Imran et al., 2018)