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Tryptophan Dioxygenase/Indole Dioxygenase (EC 1.13.11.11 and 1.13.11.52) Tryptophan dioxygenases (TDO) and indole dioxygenases (IDO) are heme-containing enzymes involved in the initial (and what is considered to be the most rate-limiting) step of the KP, catalyzing the oxidative cleavage of the indole ring of L-Trp to produce NFK (Figure 3) [117]