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In cases of hypothyroidism , the thyroid gland becomes underactive , which can significantly slow down all the functions directly affected by its hormones, and can be triggered by Hashimotos thyroiditis

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Immunofluorescence Thin sections of mouse brain and eyeball were washed with PBS, permeabilized with PBS containing 0.05% (v/v) saponin and 5% (v/v) normal goat serum for 3 min, and treated with PBS containing 1.5% normal goat serum for 1 h to block nonspecific binding

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