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Tobacco knockdown and overexpressor DHAR lines showed a substantial decrease and increase in extractable foliar activity, respectively (Chen and Gallie, DHAR2 increased the reduction state of the ascorbate pool, an effect that was observed both in control conditions and in response to aluminum stress (Yin et al., The picture that emerges from these studies is complex

Takashima M, Ichihara K, Hirata Y

Following permeabilization (0.2% Triton-X100 in PBS, 10 min, RT), endogenous peroxidases were blocked by a 10 min incubation in a 3% H 2 O 2 solution, followed by a 1 h RT incubation in PBS with 2% bovine serum albumin (BSA, Amresco, Solon, OH)

Sevin, et al