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DOI: 10.1016/j.reth.2026.101103

The samples were transferred quantitatively onto spin columns (BioEcho) and washed as follows: 0.4% SDS in PBS (3 700 l), 6 M urea in ddH 2 O (2 700 l) and PBS (3 700 l) and transferred into new 1.5-ml LoBind tubes (Eppendorf) with 2 100 l X buffer (7 M urea, 2 M thiourea in 20 mM HEPES, pH of 7.5)

Therefore, it is reasonable to assume that the BSO resistant mutations have a role in stabilizing the mature virus and likely also one or more of the capsid precursors