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After 24 h, total protein extracts were obtained from whole flies homogenized in standard lysis buffer (20 mM Tris-Cl (pH 7.5), 200 mM NaCl, 2 mM EDTA, 10% glycerol, 1% Nonidet P-40 and EDTA-free Complete Protease Inhibitor Cocktail (Roche Diagnostics))

For example, 50 mg may be divided into two 25 mg injections , or 30 mg may be divided into two 15 mg injections , administered on separate days or spaced within the same week depending on protocol preferences

Fortified nutritional yeast is the most common variety and, for the purposes of this article, is what we are referring to when we talk about nutritional yeast

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