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Description

The gating strategy is shown in Supplementary Fig

Storing the reconstituted vial at room temperature

Immunodetection was performed with primary anti-Ty1 (Diagenode, C15200054, Lot 007) or anti-tubulin (Sigma-Aldrich, clone DM1A, T6199) antibodies, followed by probing with a horseradish peroxidase-conjugated anti-mouse antibody (Dianova, DkxMu-003-FHRPX, Lot 67-83-071919)