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For pbgst-ko 2A and 2B plasmids, the 5 and 3 targeting regions of the pbgst gene were PCR-amplified using the corresponding primer pairs that produced DNA fragments as detailed in Supplementary Table S2

2023;15:e45835

LIAR hypothesis revisited: perspectives on the functional significance of mucosal eosinophils In 2010, Lee 53 and colleagues published a provocative hypothesis challenging the dogmatic view of eosinophils as solely evolving for the purpose of functioning as innate immune end-stage effector cells in type II responses