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The possible role of this extra domain in PVC remains unknown [30]

However, as it sounds like you agree, it's pretty clear that as insulin levels go down IGFBP-3 goes up...sorry, as IGF binding protein 3 goes up...maybe it's worth me taking a moment why that matters

Simultaneously, the mechanical properties of the ECM change significantly: the shear stiffness of the human nucleus pulposus increases from 12.5 kPa in Pfirrmann grade 116.5 kPa in grade V (Walter et al., 2017), collagen fibers thicken and become disorganized (Wuertz et al., 2007) (Table 1)