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FIGURE 1 To uncover relevant toxicological chemistry processes of TMS in the bloodstream, three interrelated problems need to be addressed, namely, the inherent complexity of this biological fluid (plasma contains up to 40,000 proteins and RBC cytosol up 1800 proteins), the fact that its background electrolyte corresponds to 100 mM Cl and the notion that the redox environment in which relevant processes unfold differs fundamentally between plasma (oxidizing environment) and RBCs (reducing environment)

Incubations were performed at 37 C with agar plates placed in an atmosphere of 5% CO 2 and broth cultures aerobically shaken at 170 rpm

ODTs stay at room temperature between 68 and 77 degrees Fahrenheit

Importantly, NAD+ is only one part of a complex biological system