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Consistent with this, both prior studies and our own findings in HT-1080 cells showed that treatment with latrunculin A (LatA), an actin-depolymerizing agent that induces cytoplasmic F-actin disassembly, led to a concurrent increase in nuclear F-actin assembly, suggesting a tightly coupled inverse relationship between cytoplasmic and nuclear F-actin dynamics (Figure 3O) ( FIGURE 3 pH-regulated ferroptotic nuclear F-actin assembly We serendipitously observed that the proportion of cells exhibiting nuclear F-actin assembly was markedly higher in phosphate-buffered saline (PBS) than in standard growth medium (DMEM supplemented with fetal bovine serum)

Multiple groups were compared using ANOVA

Epigenetic regulation of hepatic tumor-initiating cells